Part:BBa_K2637049:Design

EYFP cassette with TDH3 Promoter and ADH1 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 366
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1717
  

Design Notes

We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_E2030 to make the cassette which can express EYFP continously.

Source

The gene EYFP derives from Part:BBa_E2030. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.